Glucose-6-Phosphatase Assay Kit (G6Pase Assay Kit)

Price $220
Product Description: Protocol Download
Glucose-6-phosphatase (G6Pase) enzyme system is anchored in the membrane of the endoplasmic reticulum (ER) with the catalytic site facing the lumen, and converts glucose-6-phosphate to glucose and inorganic phosphate (Pi). This activity of G6Pase plays an important role in gluconeogenesis and glycogenolysis. Expression of the G6Pase gene is largely restricted to liver, kidney and to a lesser extent intestinal mucosa and pancreatic beta cells. Mutations in the catalytic subunit of G6Pase or the ER membrane transporters can influence the availability of glucose, causing glycogen storage disease (GSD) Type Ia, Ib, and Ic. G6Pase deficiency results in hypoglycemia and excessive intracellular accumulation of glucose-6-phosphate, which leads to elevated levels of lactate and uric acid. The G6Pase enzyme activity assay kit is based detection of G6Pase-mediated release of Pi, which forms phosphomolybdate with the basic dye Malachite Green (Anal. Biochem. 271:29,1999). The assay can be performed using crude liver homogenates or purified microsomal fractions. Kit components are stable for at least 1 year if stored and handled properly.

Kit Components:
G6Pase Control- 1 ml, store at -20ºC
G6Pase Reaction- 0.4 ml (after reconstitution), store at -20ºC
G6Pase Assay Solution- 30 ml (150 wells), store at 4ºC
10x Lysis Buffer: 25 ml, store at 4ºC
10x Extraction Buffer: 20 ml, store at 4ºC
TCA Solution: 4 ml, store at 4ºC

MSDS:
HCl, Malachite Green, ammonium molybdate, MES, TCA

Related Kits:
Hexokinase Assay, Glucose Assay