Glucose Assay Kit

Price $220
Product Description: Protocol Download
Glucose, being a major energy source in most eukaryotic and prokaryotic organisms, is subject to tight metabolic regulation. In humans, glucose homeostasis under different metabolic circumstances is of vital importance, and insulin regulates the concentration of glucose in the blood such that its fasting concentration normally falls within the 70 mg/dL (3.9 mM) and 100 mg/dL (5.6 mM) range. Abnormal circulating levels of glucose are associated with several pathologic states such as diabetes and obesity. Aerobic and anaerobic glucose metabolic pathways convert glucose to acetyl-CoA and lactic acid, respectively, which are differentially adopted by different tissues. Cell culture media typically contain high levels of glucose (5-25 mM), which promotes lactic acid formation and secretion in culture. The Glucose Assay Kit is based on sequential hexokinase and glucose-6- phosphate dehydrogenase reactions in a NADP+-coupled oxidation-reduction, which generates formazan exhibiting an absorption maximum at 492 nm. The Glucose Assay Kit can be used to measure the concentrations of extracellular and intracellular glucose with a detection limit of ~10 uM. The assay solution is stable for at least one year if stored and handled properly.