Nitric Oxide/Nitrite Assay Kit
Price $170
Product Description: Protocol Download
Nitric oxide (NO) is a potent endogenous vasodilator, and has a role in neurotransmission, inflammation, thrombosis, immunity, and control of cell growth and cell death. Interest in measurement of NO increased exponentially with the discovery of these versatile roles of NO in physiology as well as pathophysiology. Although NO can be measured by diazotization of sulfanilic acid, electron paramagnetic resonance, met-hemoglobin conversion, and chemiluminescence reactions (FASEB J. &:349,1993), measurement of NO in biological samples is difficult due to its low level and lability in the presence of oxygen. Nitrite is the major NO byproduct upon reaction with oxygen. Thus, the easiest method for NO measurement is the standard technique for measuring inorganic nitrite (Griess assay). This assay is based on the observation that the adduct of nitroxides and sulfanilic acid interacts with N-(1-naphthyl)ethylenediamine, generating a product that is readily monitored by spectrophotometry (Anal. Biochem. 126:131,1982). Nitrite concentrations can be obtained by comparison to a standard curve performed at the same time. The assay kit (sufficient for 500 microplate assays) is stable for one year if stored refrigerated and shielded from light.
Kit Components:
NO Assay Solution: 50 ml, store at 4ºC
Sodium Nitrite: 1 g, store at room temperature
MSDS:
phosphoric acid, N-(1-naphthyl)ethylenediamine, nitrite, sulfanilamide
Related Kits:
Lipid Peroxidation Assay, Peroxide Assay
Citation:
Kiang et al
17-DMAG diminishes hemorrhage-induced small intestine injury by elevating Bcl-2 protein and inhibiting iNOS pathway, TNF-α increase, and caspase-3 activation.
Cell Bioscience 1:21, 2011
