Peroxide Assay Kit
Price $170
Product Description: Protocol Download
Oxidative damage caused by excess reactive oxygen species (ROS) could contribute to the aging process as well as to the development of human cancer and more than 50 diseases. At the cellular levels, the oxidative damage is manifested by genetic mutation, lipid and protein peroxidation, protein cross-linking and inactivation, and changes in endogenous antioxidant levels. Major ROS include hydrogen peroxide, superoxide, and hydroxyl radical. Hydrogen peroxide (H2O2), although not a free radical, is highly reactive. The Peroxide Assay Kit is based on FOX assay, in which peroxides-mediated oxidation of Fe+2 to Fe+3 occurs in the presence of the xylenol orange dye (Free Radical Res. 37:1209-1213, 2003). The reaction product can be conveniently quantified by spectroscopy. As low as 0.1-10 uM peroxides can be detected using the assay kit in 30 min. Unlike other ROS detection kits, no protein component is incorporated into our assay kit, thus ensuring reagent stability and assay reproducibility.
Kit Components:
Peroxide Assay Solution-I: 0.5 ml, store at 4ºC
Peroxide Assay Solution-II: 50 ml, store at 4ºC
4 mM H2O2 Standard: 0.5 ml, store at 4ºC
MSDS:
sulfuric acid, xylenol orange, hydrogen peroxide, ferrous ammonium sulfate
Related Kits:
Cell Injury Assay, Lipid Peroxidation Assay, Nitric Oxide Assay
Citation:
Yoon et al
Wnt signaling regulates mitochondrial physiology and insulin sensitivity.
Genes & Development 24:1507-1518, 2010
