Sorbitol Dehydrogenase (SDH) Assay Kit                                                                                                                                                      Price

Product Description: Sorbitol dehydrogenase (SDH), found in organisms from bacteria to humans, converts sorbitol, the sugar alcohol form of
glucose, to fructose, and the zinc-dependent reaction uses NAD+ as a cofactor. Organs abundant in SDH activity include the liver, kidney, lens
and seminal vesicle. SDH and aldose reductase (AR) form the polyol pathway that interconverts glucose and fructose. Since SDH activity promotes
the formation of NADH, the redox change induced by elevated SDH may have a pathogenic role in certain disease state, making SDH a potential
therapeutic target. In addition, SDH has a close evolutionary relationship with
alcohol dehydrogenase (ADH). Our non-radioactive colorimetric SDH
assay is based on the reduction of the tetrazolium salt INT in a NADH-coupled enzymatic reaction to formazan, which is water-soluble and exhibits
an absorption maximum at 492 nm. The intensity of the red color formed is increased in the presence of increased SDH activity. The kit is stable
for at least one year under proper storage and handling conditions.

Kit Components:

SDH Assay Solution: 10 ml, store at -80ºC (for 200 wells)
25x SDH Substrate: 0.5 ml, store at -80ºC
10x Cell Lysis Solution: 25 ml, store at 4ºC

MSDS: ethanol, TX-100, DMSO, INT, acetic acid, Hepes

Related kits: Glucose Assay, Glucose-6-phosphate Assay, ADH Assay, GAPDH Assay
Site Map
Biomedical Research Service
& Clinical Application
Alphabetical Listing