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Plasmid DNA is purified from DH5a strain using our Maxi Plasmid Purification Kit.
Service fee for each plasmid is $160 (20 ug DNA).

Promoter-less Reporter Vectors: Your favorite gene promoter can be inserted into these reporter vectors
p-lacZ         beta-galactosidase reporter        
p-luc          firefly luciferase reporter        
p-cat          chloramphenicol acetyl transferase
p-EGFP         enhanced green fluorescence protein

Promoter-Reporter Vectors: Expression is driven by constitutive eukaryotic promoters/enhancers
pActin-lacZ    beta-galactosidase reporter        
pCMV-lacZ      beta-galactosidase reporter        
pRSV-lacZ      beta-galactosidase reporter        
pSV-lacZ       beta-galactosidase reporter        
pCMV-luc       firefly luciferase reporter        
pSV-luc        firefly luciferase reporter        
pTK-luc        firefly luciferase reporter        
pActin-cat     CAT reporter                       
pSV-cat        CAT reporter                       
pTK-cat        CAT reporter                       
pCMV-EGFP      green fluorescence protein reporter

Eukaryotic Expression Vectors: Your favorite gene can be inserted into the vectors for overexpression
pSV            Simian Virus 40 promoter          
pMSV           Moloney sarcoma virus promoter     
pCMV           Cytomegalovirus promoter
pADH           Alcohol dehydrogenase promoter  
pSFFV-neo      neomycin selection marker      
pCMV-HA        for HA tag fusion at the N-terminus

GAL4 Fusion Vectors: These vectors encode a truncated yeast GAL4 DNA-binding domain (1-147), which can be fused
in-frame with your favorite gene. Three different fusion sequences were available (DB1, DB2, DB3) for expression in
higher eukaryotes
pGAL4-DB1      GAL4 fusion protein              
pGAL4-DB2      GAL4 fusion protein              
pGAL4-DB3      GAL4 fusion protein         

Minimum Promoter Vectors: These reporter vectors contain either cat or luc reporters driven by a minimal TATA box or
a minimal TATA box linked to an upstream activating sequence (typically multiple copies of a transcription factor
binding site)
pTATA-luc      luciferase reporter driven by a minimal TATA box       
pGAL4-TATA-luc luciferase reporter driven by a minimal TATA box and multiple GAL4 binding sites              
pGAL4-TATA-cat cat reporter driven by a minimal TATA box and multiple GAL4 binding sites
pSRF-TATA-luc  luciferase reporter driven by a minimal TATA box and an SRF binding site
pHSF-TATA-luc  luciferase reporter driven by a minimal TATA box and multiple HSF binding sites
pNFkb-TATA-luc luciferase reporter driven by a minimal TATA box and multiple NFkb binding sites
pE12-TATA-luc  luciferase reporter driven by a minimal TATA box and multiple E12 binding sites   

Yeast Expression Vectors:
pYBD           expression of a fusion protein containing the GAL4 DNA-binding domain
pYAD           expression of a fusion protein containing the GAL4 activation domain
pRS313         yeast centromere vector with his3 marker
pRS314         yeast centromere vector with trp1 marker
pRS315         yeast centromere vector with leu2 marker
pRS316         yeast centromere vector with ura3 marker

Bacterial Expression vectors:
pGEX           Glutathione S-transferase (GST) fusion at the N-terminus
pET3a          for protein overexpression
pET3b          for protein overexpression
pET3c          for protein overexpression
pET3d          for protein overexpression
pET14b         His tag fusion at the N-terminus
pEGFP          expression of EGFP in bacteria
pSP64          bacterial expression

Eukaryotic EGFP Vectors:
pCMV-EGFP-neo  expression of EGFP and neomycin selection marker
pEGFP-C1       expression of C-terminal EGFP fusion in higher eukaryotes
pCMS-EGFP      coexpression with EGFP in higher eukaryotes
pHyg-EGFP      expression of hygromycin-EGFP fusion protein in higher eukaryotes

General Cloning vectors:
pUC8
pUC19
pBR322
pGEM-3z
pGEM-7z
pBluescript
pTZ-19R
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