UDP-Glucose Dehydrogenase (UGDH) Assay Kit
Price $260
Product Description: Protocol Download
UDP-glucose dehydrogenase (UGDH) is an essential enzyme that converts UDP-glucose (UDPG) into UDP-glucuronic acid (UDPGA). The enzyme uses the cofactor NAD⁺ to drive a two-step oxidation of UDPG. The product UDPGA is an anabolic building block for the gel-like substances vital for cellular/organ function and wound healing, such as hyaluronic acid and chondroitin sulfate, which are components of glycosaminoglycans (aka mucopolysaccharides). UDPGA also participates in conjugation of endogenous metabolites and exogenous compounds for excretion such as various drugs and steroids, i.e., glucuronidation, which is a critical Phase II liver detoxification process to make compounds more soluble for excretion. A prime example is the hepatic conjugation of bilirubin, the failure of which process leads to jaundice. In plants and most animal species, UDPGA is the starting point for making ascorbic acid (vitamin C). An increased expression of UGDH has been linked to the spread of breast and ovarian cancer because the enzyme activity promotes the survival of tumor-initiating cells (TICs) presumably through alterations of the ECM, making it hard for immune cells to eradicate the TICs. Thus, UGDH is considered a highly promising target for cancer therapy. The UGDH enzyme activity assay kit is based on NADH- and diaphorase-mediated reduction of INT, leading to the formation of the chromogenic compound INT-formazan, which is detected by absorbance at 492 nm. The kit is stable for many years if stored and handled properly.
Kit Components:
UGDH Assay Solution: 5 ml, store at -80℃
20x UGDH Substrate: 0.25 ml, store at -80℃
10x Sample Buffer: 25 ml, store at 4℃
Related Kits:
HK/GK Assay, GFAT Assay
