Thiolase II (ACAT) Assay Kit
Acetoacetyl-CoA Thiolase or Acetyl-CoA Acetyltransferase
Price $270
Product Description: Protocol Download
Two different types of thiolases are present in eukaryotes and in prokaryotes: 3-ketoacyl-CoA thiolase (aka thiolase I) and acetoacetyl-CoA thiolase (aka thiolase II). 3-ketoacyl-CoA thiolase has a broad substrate specificity and participates in degradative pathways such as fatty acid beta-oxidation. Acetoacetyl-CoA thiolase, or acetyl-CoA acetyltransferase (ACAT), catalyzes the reversible transfer of an acetyl group from one acetyl-CoA to another acetyl-CoA, forming acetoacetyl-CoA, which is an intermediate in the production of ketone bodies and in the biosynthesis of cholesterol and steroids. The activity of thiolase II is regulated by various factors such as the concentration of acetyl-CoA as high levels of acetyl-CoA promote the catalytic activity of the enzyme. The enzyme is also controlled by post-translational modifications and protein interactions. Since dysregulation of thiolase can cause metabolic disorders, the enzyme has become a target for drug interventions. The thiolase II assay kit is based on CoA-dependent reduction of INT to formazan, which exhibits an absorption maximum at 492 nm and allows for measurement of acetoacetyl-CoA thiolase activity present in cell/tissue lysates. Kit components are stable for at least one year if stored and handled properly.
Kit Components:
Thiolase II Assay Solution: 5 ml, store at -80°C (for 100 wells)
10x Acetyl-CoA: 0.5 ml, store at -80ºC
10x Cell Lysis Solution: 25 ml, store at room temperature
Related Kits:
FAO Assay, CPT1 Assay, BHB Assay, BDH Assay
