Alanine Aminotransferase Assay Kit (ALT Assay Kit)

Price $220
Product Description: Protocol Download
Alanine aminotransferase (ALT) is a member of the transaminase family of enzymes. The enzyme is previously referred to as glutamate pyruvate transaminase (GPT), which indicates its biological function in intermediary metabolism of glucose and protein through catalyzing the transfer of an amino group between alanine and glutamate. ALT is present at the highest level in the liver with smaller amounts of the enzyme in the heart, muscle, and kidney, and as such its measurement constitutes part of the liver function tests. While marked elevation in serum ALT may signal acute liver damage even prior to manifestation of the disease symptom, it can also be associated with cardiovascular and muscle diseases. Assessment of the ratio of AST to ALT also provides clinically useful information. The colorimetric ALT assay is based on the reduction of the tetrazolium salt INT in a NADH-coupled enzymatic reaction to formazan, which is water-soluble and exhibits an absorption maximum at 492 nm. The intensity of the red color formed is increased in the presence of increased ALT activity. The kit is stable for at least one year if handled and stored properly.

Kit Components:
ALT Substrate: 2 ml, store at -80°C
TA Assay Solution: 5 ml (for 100 wells), store at -80°C
10x Cell Lysis Solution: 25 ml, store at room temperature

MSDS:
TX-100, DMSO, INT, Tris

Related Kits:
AST Assay, LDH Assay

Citation:
Zhu et al
Enhanced anticancer activity of nanopreparation containing an MMP2-sensitive PEG-drug conjugate and cell-penetrating moiety
PNAS 110(42):17047-17052, 2013

Essex et al
Phospholipid-modified PEI-based nanocarriers for in vivo siRNA therapeutics against multidrug-resistant tumors
Gene Therapy 22:257-266, 2015