TMB ELISA Substrate(for ELISA application)
Price $100
Product Description: Protocol Download
One of the most widely used enzymes for chromogenic detection in immunoassays is horseradish peroxidase (HRP), a highly stable enzyme (Clin. Chim. Acta 81:1,1977). The compound 3,3′,5,5′-tetramethylbenzidine (TMB) has emerged as the safest colorigenic substrate of HRP exhibiting excellent sensitivity (Tetrahedron 30:3299,1974). HRP in the presence of peroxide converts TMB to a bluish reaction product that can be read spectrophotometrically at 370 or 655 nm. The blue end product upon acidification generates a bright yellowish color enabling sensitive HRP detection and quantification by absorbance at 450 nm. This application has become widely used in Enzyme-Linked Immunosorbent Assay (ELISA) because of its lowest detection limit compared to other reporter enzymes and substrate systems. The working substrate solution needs to be prepared fresh by mixing TMB Solution and Peroxide Solution. The ELISA substrate solution is stable for ~1 year if stored and handled properly.
Kit Components:
TMB Solution: 120 ml, store at room temperature
Peroxide Solution: 0.3 ml, store at 4C
TMB Stop: 60 ml, store at room temperature
MSDS:
TMB, DMSO, Hydrogen peroxide, Hydrochloric acid
Citation:
Mastri et al
Activation of Toll-like receptor 3 amplifies mesenchymal stem cell trophic factors and enhances therapeutic potency.
Am J Physiology 303:C1021-1033, 2012
