D-Lactate Assay Kit

Price $250
Product Description: Protocol Download
D-lactate accumulation in body fluids indicates bacterial infection or metabolic abnormality, and detection of D-lactate is therefore of clinical value. Unlike L-lactate, which is present at mM levels in the circulation, D-lactate is usually too low to be detected in healthy subjects. D-Lactate can be derived from methylglyoxal, a major source of dicarbonyl oxidative stress, through glyoxalase 1 and 2 (GLO1 and GLO2), and further converted to pyruvate by lactate dehydrogenase-D (LDH-D), thus completing the methylglyoxal detoxification pathway. The D-Lactate Assay Kit is based on the reduction of the tetrazolium salt INT in a NADH-coupled enzymatic reaction to formazan, which exhibits an absorption maximum at 492 nm. The D-Lactate Assay kit can measure the concentration of D-lactate present in serum or plasma with a detection limit of 1-5 uM. Samples need to be deproteinized prior to assay. The assay solution is stable for at least one year if stored properly. Keep solutions at -80ºC for long term storage (more than 1 month).