Glucose-6-Phosphate Assay Kit

Price $220
Product Description: Protocol Download
Glucose has only one principle fate upon entering the cell: phosphorylation to glucose-6-phosphate (G6P) by hexokinase or glucokinase. The phosphorylation of glucose with ATP is irreversible under the conditions existing in cells and serves to trap the glucose within the cytosol of the cell. G6P is an intermediate in almost every pathway that uses glucose, including glycolysis, the pentose phosphate pathway and the pathway for glycogen synthesis. The ATP expenditure at the beginning of the glycolytic pathway is sometimes called “priming the pump” because it gets the pathway going. Glucose-6-phosphatase, not present in muscle or brain, enables the liver to release glucose into the circulation. The colorimetric G6P assay is based on the reduction of the tetrazolium salt INT in a NADPH-coupled enzymatic reaction, and can detect as low as 1 – 10 uM G6P. The INT reaction product is water-soluble and exhibits an absorption maximum at 492 nm. The intensity of the red color formed is increased in response to increased G6P concentration. The assay solution should be kept at -80ºC, and will remain stable indefinitely at -80ºC.