Ethanol Assay Kit

Price $230
Product Description: Protocol Download
Alcoholic drinks vary widely in ethanol content, and the concentration of alcohol is usually marked as the percentage of alcohol by volume (ABV) or as proof (e.g., 100 proof = 50% ABV). Blood alcohol content (BAC) is most commonly used in medical or legal arenas. Serious intoxication can be observed with a BAC reading of 0.2%, and 0.4% is the accepted LD50 for adult humans. Being a central nervous system depressant, ethanol has profound psychoactive effects in sub-lethal doses. The assay kit takes advantage of the narrow specificity of yeast alcohol dehydrogenase (ADH), and thus primarily detects ethanol, although other straight chain primary alcohols and aldehydes also react with the assay solution. The kit is designed to measure ethanol in serum, urine, food beverages, and culture medium samples. Assay is based on the reduction of INT in a NADH-coupled reaction to formazan, which exhibits an absorption maximum at 492 nm. The intensity of the red color formed is proportional to sample ethanol concentrations with a linear range of 0.01 – 0.2% ethanol. Since the assay reaction is enzymatic in nature, non-specific interference may occur with certain complex samples. The assay solution should be kept at -80ºC for longer-term storage (more than 1 month).