Cell Injury Assay Kit

Price $220
Product Description: Protocol Download
The kinetic determination of the cellular enzyme lactate dehydrogenase (LDH)released upon cell damage or lysis is a sensitive, precise, and convenient method for the studies of apoptosis and cytotoxicity reactions (J. Immunol. Methods 64:313,1983). The inexpensive, non-radioactive colorimetric LDH assay provides a superb alternative to 51Cr release assays (Immunology 14:181,1968), which often suffer from higher background levels. The Cell Injury Assay Kit is based on the reduction of the tetrazolium salt INT in a NADH-coupled enzymatic reaction to formazan, which is water-soluble and exhibits an absorption maximum at 492 nm. Since the intensity of the red color formed is proportional to the number of damaged or lysed cells, the assay has been widely used for the detection and quantification of apoptosis as well as necrosis (Exp. Cell Res. 226:372, 1996) (see Cell Viability/Proliferation Assay Kit). The superior sensitivity of the non-radioactive assay kit allows the assay to be completed within 30 min with minimal pipetting steps. Each kit is sufficient for approximately 200 assays using the 96-well format. See related product Lactate Assay Kit. The method is adaptable to automation.