Urea Assay Kit
Price $190
Product Description: Protocol Download
Urea is the major organic component of human urine, representing the end waste product of protein metabolism. Amino acids are metabolized and converted to ammonia, which at high levels is toxic to cells. The Urea cycle, which occurs in the liver, mediates the removal of ammonia as urea in the amount of 10 to 20 g per day. A defective urea cycle may therefore cause hyperammonemic encephalopathy and brain injury. A failure of ureagenesis can occur due to acquired disease, e.g., cirrhosis secondary to alcoholism, or secondary to a congenital enzymopathy. Since urea is carried by blood to the kidneys for elimination from the body, high levels of urea in the blood may indicate renal failure. Elevated blood urea may also be caused by use of corticosteroids, dehydration, or congestive heart failure. Decreased blood urea levels can occur in response to liver disease, malnutrition, or over-vigorous parenteral fluid therapy. Urea determination is thus useful for assessing the kidney function of patients. The Urea Assay Kit is non-enzymatic, and is based on the modified Jung method (Biochem Cell Biol. 87:541–544, 2009), which involves a specific condensation reaction of o-phthalaldehyde with urea. The formed carbonium ion further reacts with a coloring compound, thus enabling measurement of urea by light absorbance at 430-450 nm.
Kit Components:
UR Reagent 1: 5 ml (for 200 wells), store at RT
UR Buffer: 20 ml, store at RT
Primaquine: 20 mg, store at RT
UR Reagent 2: 0.1 ml, store at RT
Urea: 100 mg, store at RT
MSDS:
boric acid, o-phthaldialdehyde, primaquine bisphosphate, sulfuric acid
Related Kits:
Ammonia Assay, AST Assay, ALT Assay
