Pyruvate Assay Kit 

Price $290
Product Description: Protocol Download
Pyruvate metabolism is a central biochemical hub that connects glycolysis to cellular energy production and biosynthetic pathways. Pyruvate is metabolized aerobically to acetyl-CoA by pyruvate dehydrogenase complex, which represents the rate-limiting step in eukaryotic pyruvate metabolism. Under an anaerobic condition or in the presence of an increased glycolytic flux, pyruvate can be converted into lactate by lactate dehydrogenase, i.e., homolactic fermentation. Conversion of pyruvate to lactate is obligatory for bacteria, serving to regenerate NAD+, and is enhanced in eukaryotic cells when mitochondrial function is impaired. LDH-A-mediated conversion of pyruvate into lactate by cancer cells in the presence of adequate oxygen supply constitutes the central concept of the Warburg effect. In yeast, alcoholic fermentation of pyruvate generates ethanol, which is mediated by pyruvate decarboxylase and alcohol dehydrogenase. Pyruvate is also converted to oxaloacetate (OAA) by an anaplerotic reaction mediated by pyruvate carboxylase and OAA can serve as a critical gluconeogenic substrate in the fasted state. The Pyruvate Assay Kit is based on pyruvate oxidase-mediated production of hydrogen peroxide and detection of hydrogen peroxide using Trinder’s reagent by absorbance at 590 nm. The detection sensitivity is 1-10 mM pyruvate. Kit components are stable for at least one year if stored and handled properly.

Kit Components:
Pyruvate Solution-I: 0.25 ml (for 100 wells), store at -20ºC
Pyruvate Solution-II: 5 ml (for 100 wells), store at -20°C
20 mM Pyruvate: 0.2 ml, store at -20ºC (short term) or -80ºC (long term)
H2O2 Assay Solution: 2 ml, store at -20ºC

Related Kits:
Lactate Assay, Glucose Assay, G6P Assay,  PDC Assay

SDS:
DMSO, HDAOS, 4-Aminoantipyrine