Product Description:

The detergent compatible Protein Assay Kit combines two famous reactions- the Biuret reaction and the Smith reaction. The Biuret reaction depicts the reduction of cupric ion (Cu2+) to cuprous ion (Cu1+) by protein in an alkaline solution. The intensity of the reaction is proportional to the number of peptide bonds (at least 2 peptide bonds) and therefore to the number of protein molecules present in the sample solution. The Smith reaction depicts the chelation of each Cu1+ ion by two molecules of bicinchoninic acid (BCA), resulting in an intense purple-colored product that absorbs light at 562 nm (Anal. Biochem. 150: 76–85, 1985), which can be measured with a standard laboratory spectrophotometer or microplate reader. Unlike the dye-based protein assay system, the BCA-based protein assay system is compatible with detergent-containing protein samples. However, the BCA assay is incompatible with most divalent ions. Prepare fresh working solution by mixing 50 parts of BCA Solution with 1 part of Copper Solution (50:1). The Protein Assay System has a broad working range of 20 - 2000 ug/ml proteins. The assay kit also includes 1 ml of 100 mg/ml sterilized BSA dissolved in distilled water to be diluted for use as protein standard. The assay kit is stable for at least one year under proper storage and handling conditions.

Kit Components:

BCA Solution: 200 ml, store at room temperature

Copper Solution: 5 ml, store at room temperature

100 mg/ml Bovine Serum Albumin (BSA): 1 ml, store at -20ºC

MSDS:

NaOH, BCA, Potassium sodium tartrate, Cupric sulfate

Related Kits:

Protein Assay Kit (EDTA/Mg+2 compatible), DAB Substrate kit, TMB Substrate kit, BCIP Substrate kit

Citation:

Martin et al

Tissue-nonspecific alkaline phosphatase as a target of sFRP2 in cardiac fibroblasts

Am J Physiol Cell Physiol, 309:C139, 2015