NAD+/NADH Assay Kit
Price $240
Product Description: Protocol Download
In metabolism, the compound nicotinamide adenine dinucleotide accepts or donates electrons in redox reactions, forming the NAD+/NADH redox pair with a redox potential of -0.32 volts. The NAD+/NADH ratio is an important measurement that reflects both the metabolic and health states of cells. Key enzymes regulating the NAD+/NADH ratio are those dehydrogenases involved in fuel metabolism (such as lactate dehydrogenase and pyruvate dehydrogenase) and electron transport chain complexes (such as NADH dehydrogenase). Non-redox roles of NAD+ have received increasing attention in recent years. For instance, NAD+ is consumed in ADP-ribosylation reactions mediated by ADP-ribosyltransferase and poly(ADP-ribose) polymerase. Sirtuins, a group of NAD-dependent deacetylases, also consume NAD+, and this enzymatic activity appears to be critically involved in the aging of vertebrates and invertebrates. Degradation of NAD+ is also accelerated during inflammation and in the aging tissue due to increased expression of CD38. The NAD+/NADH Assay Kit is based on the reduction of the tetrazolium salt INT in a NADH-coupled enzymatic reaction to formazan, which is water-soluble and exhibits an absorption maximum at 492 nm (detection limit ~5 uM). Note that the sample NADH concentration, which is overestimated in the assay, should be used as reference only. Components of the assay kit are stable for at least one year if stored and handled properly.
Kit Components:
NADH Assay Solution: 10 ml, store at -80°C (for 100 wells)
Reagent 1: 1 ml, store at -20°C
Reagent LA: 100 ul, store at -20°C
Reagent LD: 60 ul, store at 4°C
Reagent DI: 1.5 mg, store at -20C
NADH: 10 mg, store at -20°C
NADH Buffer: 4 ml, store at 4°C
Related Kits:
L-Lactate Assay, ATP Assay, Fructose Assay, CD38 Assay
