Product Description:
Lactate dehydrogenase (LDH) is an oxidoreductase which catalyzes the interconversion of lactate and pyruvate. It consists of 4 subunits which may be of 2 different types: M (muscle) and H (heart), also known as A and B respectively. Five different isoenzymes are therefore possible, depending on the subunit composition. The LDH staining kit is based on the reduction of a tetrazolium salt to a formazan product, which is water insoluble and exhibits an intense dark blue color. Since the intensity of the dark blue color is proportional to LDH activity, the staining kit can be used to delineate cell/tissue LDH isoform activity by either gel assay or in situ staining (see gel on right).
#Lactate Dehydrogenase Staining
Kit Components:
LDH Staining Solution: 40 ml, store at -80°C
10x Cell Lysis Solution: 25 ml, store at 4ºC
Loading Solution: 0.5 ml, store at 4ºC
SDS:
TX-100, MTT, Hepes, DMSO
Related Kits:
HK Enzyme Assay, GAPDH Enzyme Assay, LDH Enzyme Assay, Glucose Assay, D-Lactate Assay, PDH Enzyme Assay,
G6PD Enzyme Assay, Pyruvate Assay, NAD+ assay,
L-Lactate Assay
