Product Description:
The bacterial enzyme beta-galactosidase (LacZ) is a widely used reporter enzyme, the activity of which can be conveniently measured spectrophotometrically (J. Med. Chem. 7:574,1964). The beta-Gal assay kit utilizes the spectroscopically active compound o-nitrophenyl-beta-D-galactopyranoside (ONPG) for quantitative measurements of Gal activity in cells (higher eukaryotes, yeast, or bacteria) transfected or transformed with Gal reporter DNA (see beta-Galactosidase Staining Kit). The enzyme converts ONPG to o-nitrophenol, which exhibits an extinction coefficient of 4,500 M-1cm-1 at 414 nm. Each assay kit includes 20 ml of optimized ONPG Substrate Solution sufficient for 200 wells in microplate formats. Cell Lysis Solution is compatible with luciferase (see Luciferase Assay Kit) and CAT enzyme assays permitting flexibility and maximum sensitivity. Substrate is stable for at least one year if handled and stored properly. The method is adaptable to automation.
#LacZ
Kit Components:
LacZ Assay Solution: 20 ml, store at -20ºC (for 200 wells)
10x Cell Lysis Solution: 25 ml, store at 4ºC
LacZ Stop Solution: 10 ml, store at room temperature
SDS:
TX-100, ONPG, sodium bicarbonate, 2-mercaptoethanol, chloroform
Related Kits:
ALP Assay, LacZ Staining, Luciferase Assay
Citation:
Phenotypic changes of adult porcine mesenchymal stem cells induced by prolonged passaging in culture.
J Cell Physiology 205:194-201, 2005
