Product Description: Price

Αlpha-ketoglutarate (oxoglutarate) dehydrogenase complex (aKGDC), a Krebs Cycle enzyme complex, catalyzes the oxidative decarboxylation of alpha-ketoglutarate to succinyl-CoA with concomitant production of NADH. The enzyme complex together with pyruvate dehydrogenase complex (PDC) and branched-chain ketoacid dehydrogenase complex (BCKDC) belong to the alpha-ketoacid dehydrogenase complex family. Each of these enzyme complexes is a multiunit complex consisting of unique E1 (alpha-ketoacid dehydrogenase) and E2 (dihydrolipoyl transacetylase) subunits as well as an identical E3 subunit (dihydrolipoyl dehydrogenase), which catalyzes the transfer of two hydrogen atoms from dihydrolipoic acid to its prosthetic group FAD and restores oxidized lipoic acid. The reduced FADH2 of the E3 subunit then transfer H+ to NAD+ to form NADH. aKGDC represents a major modulator of oxidative phosphorylation and Krebs Cycle flux, and is involved in the metabolic remodeling following a cancer cell’s transformation in bioenergetic requirements. Deficiency of the enzyme complex has been described as a variant form of maple syrup urine disease (MSUD) as a result of deficiency of the E3 component, leading to PDC and BCKDC deficiencies. This non-radioactive colorimetric aKGDC assay is based on the reduction of the tetrazolium salt INT in a NADH-coupled enzymatic reaction to formazan, which is water-soluble and exhibits an absorption maximum at 492 nm. The intensity of the red color formed is increased in the presence of increased aKGDC activity. The kit is stable for many years under proper storage and handling conditions.

#KrebsCycle #alpha-KetoglutarateDehydrogenaseComplex #branchedchainaminoacid

#MSUD #MapleSyrupUrineDisease

Kit Components:

aKGDC Assay Solution: 5 ml (for 100 wells), store at -80ºC

20x Cofactors: 0.25 ml, store at -80ºC

20x KGDC Substrate: 0.25 ml, store at -80ºC

10x Lysis Buffer: 25 ml, store at 4ºC

10x Extraction Buffer: 20 ml, store at 4ºC

SDS:

DMSO, INT, Tris

Related Kits:

PDC Assay, BCKDC Assay

Citation:

Sabatini et al.

The proneural transcription factor Atoh1 promotes odontogenic differentiation in human dental pulp stem cells (DPSCs)

BMC Mol Cell Biology 26:1-10, 2025