Product Description:

G6Pase is anchored in the membrane of the ER with the catalytic site facing the lumen, and converts glucose-6-phosphate to glucose and inorganic phosphate (Pi). This activity of G6Pase plays an important role in gluconeogenesis and glycogenolysis. Expression of the G6Pase gene is largely restricted to liver, kidney and to a lesser extent intestinal mucosa and pancreatic beta cells. Mutations in the catalytic subunit of G6Pase or the ER membrane transporters can influence the availability of glucose, causing GSD Type Ia, Ib, and Ic. G6Pase deficiency results in hypoglycemia and excessive intracellular accumulation of glucose-6-phosphate, which leads to elevated levels of lactate and uric acid. The G6Pase enzyme activity assay kit is based detection of G6Pase-mediated release of Pi, which forms phosphomolybdate with the basic dye Malachite Green (Anal. Biochem. 271:29,1999). The assay can be performed using crude liver homogenates or purified microsomal fractions. Kit components are stable for at least 1 year if stored and handled properly.

#Glucose-6-phosphatase​

Kit Components:

G6Pase Control- 1 ml, store at -70ºC

G6Pase Reaction- 0.4 ml (after reconstitution), store at -70ºC

G6Pase Assay Solution- 30 ml (150 wells), store at 4ºC

10x G6Pase Buffer: 10 ml, store at 4ºC

TCA Solution: 2 x 1 ml (corrosive to metals and tissue), store at 4ºC

SDS:

HCl, Malachite Green, ammonium molybdate, MES, TCA

Related Kits:

HK Enzyme Assay, GAPDH Enzyme Assay, LDH Enzyme Assay,

L-Lactate Assay, D-Lactate Assay, PDH Enzyme Assay,

Glucose Assay