Creatine Kinase Assay Kit (CK Assay Kit)

Price $240
Product Description: Protocol Download
Creatine kinase (CK) catalyzes the reversible conversion between creatine and phosphocreatine (PCr), which is coupled to the equilibrium between ATP and ADP in the cytosol and mitochondria, constituting an important intracellular energy shuttling system mediated by mitochondrial and cytosolic CK isoforms. While mitochondrial CK forms PCr from mitochondrial ATP, cytosolic CK, using PCr, regenerates ATP consumed in metabolic reactions and mechanical work. CK is thus an important enzyme for tissues that utilize ATP rapidly. There are five different CK isoforms: three cytosolic (CK-MM, CK-BB and CK-MB) and two mitochondrial (ubiquitous and sarcomeric), which exhibit differential tissue distribution. Clinically, assay of serum CK level is utilized in the diagnosis and monitoring of myocardial infarction, myopathies and cerebrovascular accidents. Serum CK activity may exhibit an inverse relationship with thyroid activity. The sensitive enzyme activity assay kit can measure CK activity present in serum/plasma and crude cell/tissue extracts. Luciferase-based detection of ATP produced by CK reaction allows fast, sensitive and quantitative assay of total CK activity. Measurement of ATP requires a luminometer or scintillation counter. Kit components are stable for many years if stored and handled properly.

Kit Components:
CK Reaction- 0.25 ml, store at -80ºC
CK Control- 0.25 ml, store at -80ºC
TCA Stop- 1 ml, store at 4ºC
10x Sample Buffer- 25 ml, store at 4ºC
ATP Assay Solution- 2×10 ml (100 assays), store at -80ºC

MSDS:
TX-100, luciferin, Tris, DTT

Related Kits:
ADK Assay, NDK Assay