Product Description:

Alkaline phosphatase (ALP) and horseradish peroxidase (HRP) are two reporter enzymes widely used in immunoassays due to their stability. In addition, ALP is a sensitive marker of embryonic stem cells and induced pluripotent stem cells. 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) is a synthetic chromogenic substrate, which upon cleavage by alkaline phosphatase is converted to 5-bromo-4-chloro-3-indole and inorganic phosphate. In the presence of a tetrazolium salt such as NBT or MTT, 5-bromo-4-chloro-3-indole is further converted to an insoluble dark blue diformazan precipitate, thus enabling its use for sensitive ALP-based application and detection of pluripotent stem cells. The working solution can be used for ALP-based in situ immunostaining and Western blotting. The kit is sufficient for preparing 200 ml of ALP staining solution.

#BCIP Substrate

Kit Components:

20x ALP Buffer: 10 ml, store at room temperature

50x BCIP: 4 ml, store at 4ºC

50x Tetrazolium: 4 ml, store at 4ºC

MSDS:

BCIP, MTT, Tris, dimethylformamide, ethanol

Related Kits:

DAB Substrate kit, TMB Substrate kit, Protein Assay Kit (EDTA/Mg2+ compatible), Protein Assay Kit/D (detergent compatible)