Product Description:
Alkaline phosphatase (ALP) and horseradish peroxidase (HRP) are two reporter enzymes widely used in immunoassays due to their stability. 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) is a synthetic chromogenic substrate, which upon cleavage by alkaline phosphatase is converted to 5-bromo-4-chloro-3-indole and inorganic phosphate. In the presence of a tetrazolium salt such as NBT or MTT, 5-bromo-4-chloro-3-indole is further converted to an insoluble dark blue diformazan precipitate, thus enabling its use for sensitive ALP-based protein detection. The working solution can be used for ALP-based in situ immunostaining and Western blotting. The kit is sufficient for preparing 200 ml of ALP staining solution.
#Alkaline Phosphatase Staining
Kit Components:
20x ALP Buffer: 10 ml, store at room temperature
50x BCIP: 4 ml, store at -20ºC
50x Tetrazolium: 4 ml, store at -20ºC
SDS:
BCIP, MTT, Tris, dimethylformamide, ethanol
Related Kits:
DAB Substrate kit, TMB Substrate kit, Protein Assay Kit (EDTA/Mg2+ compatible), Protein Assay Kit/D (detergent compatible)
Citation:
Martin et al
Tissue-nonspecific alkaline phosphatase as a target of sFRP2 in cardiac fibroblasts
Am J Physiol Cell Physiol, 309:C139, 2015
