LacZ Assay Kit Price
Product Description: The bacterial enzyme beta-galactosidase (LacZ) is a widely used reporter enzyme, the activity of which can be
conveniently measured spectrophotometrically (J. Med. Chem. 7:574,1964). The beta-Gal assay kit utilizes the spectroscopically active compound
o-nitrophenyl-beta-D-galactopyranoside (ONPG) for quantitative measurements of Gal activity in cells (higher eukaryotes, yeast, or bacteria)
transfected or transformed with Gal reporter DNA (see beta-Galactosidase Staining Kit). The enzyme converts ONPG to o-nitrophenol, which
exhibits an extinction coefficient of 4,500 M-1cm-1 at 414 nm. Each assay kit includes 20 ml of optimized ONPG Substrate Solution sufficient for
200 wells in microplate formats. Cell Lysis Solution is compatible with luciferase (see Luciferase Assay Kit) and CAT enzyme assays permitting
flexibility and maximum sensitivity. Substrate is stable for at least one year if handled and stored properly. The method is adaptable to automation.
Kit Components:
LacZ Assay Solution: 20 ml, store at -20ºC (for 200 wells)
10x Cell Lysis Solution: 25 ml, store at 4ºC
LacZ Stop Solution: 10 ml, store at room temperature
MSDS: TX-100, ONPG, sodium bicarbonate, 2-mercaptoethanol, chloroform
Related kits: ALP Assay, LacZ Staining, Luciferase Assay
Citation:
Phenotypic changes of adult porcine mesenchymal stem cells induced by prolonged passaging in culture.
J Cell Physiology 205:194-201, 2005
Product Description: The bacterial enzyme beta-galactosidase (LacZ) is a widely used reporter enzyme, the activity of which can be
conveniently measured spectrophotometrically (J. Med. Chem. 7:574,1964). The beta-Gal assay kit utilizes the spectroscopically active compound
o-nitrophenyl-beta-D-galactopyranoside (ONPG) for quantitative measurements of Gal activity in cells (higher eukaryotes, yeast, or bacteria)
transfected or transformed with Gal reporter DNA (see beta-Galactosidase Staining Kit). The enzyme converts ONPG to o-nitrophenol, which
exhibits an extinction coefficient of 4,500 M-1cm-1 at 414 nm. Each assay kit includes 20 ml of optimized ONPG Substrate Solution sufficient for
200 wells in microplate formats. Cell Lysis Solution is compatible with luciferase (see Luciferase Assay Kit) and CAT enzyme assays permitting
flexibility and maximum sensitivity. Substrate is stable for at least one year if handled and stored properly. The method is adaptable to automation.
Kit Components:
LacZ Assay Solution: 20 ml, store at -20ºC (for 200 wells)
10x Cell Lysis Solution: 25 ml, store at 4ºC
LacZ Stop Solution: 10 ml, store at room temperature
MSDS: TX-100, ONPG, sodium bicarbonate, 2-mercaptoethanol, chloroform
Related kits: ALP Assay, LacZ Staining, Luciferase Assay
Citation:
Phenotypic changes of adult porcine mesenchymal stem cells induced by prolonged passaging in culture.
J Cell Physiology 205:194-201, 2005
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