Fructose Assay Kit
(for glucose-free samples)

Price $220
Product Description: Protocol Download
Fructose is the sweetest naturally occurring carbohydrate widely added to beverages as a sweetener in the form of high-fructose corn syrup (HFCS). Chronic consumption of HFCS-containing drinks has been linked to increased health risks contributing to the development of metabolic syndrome, which is marked by type 2 diabetes (insulin resistance), overweight, nonalcoholic fatty liver disease (NAFLD), and cardiovascular disease. Excessive dietary fructose can decrease the expression of endothelial nitric oxide synthase (eNOS) and therefore diminished NO, causing hypertension and hyperuricemia. Fructose metabolism begins with fructokinase-mediated phosphorylation of fructose in the liver, generating fructose-1-phosphate, which is subsequently cleaved by aldolase B. Since the phosphorylation reaction is not feedback regulated, high-fructose intake can potentially lead to ATP depletion. Fructose measurements can thus provide valuable information regarding metabolic integrity in the context of human diets, which is vital for the management of genetic diseases such as hereditary fructose intolerance (HFI) and fructose malabsorption. The assay kit quantifies fructose present in culture medium, serum/plasma, and urine by a simple measurement of light absorbance (O.D.) at 492 nm contributed by the reduction of the tetrazolium salt INT, and exhibits a detection limit of ~10 uM fructose and glucose. Kit components are stable for at least one year if stored and handled properly.