YY1 (Ying-Yang 1) Price
BACKGROUND: The multi-functional transcription factor and nuclear matrix protein YY1 (Ying-Yang 1) was first discovered in 1991 for its ability to
dually trans-activate or trans-repress gene promoters. YY1 has been studied extensively across multiple disciplines. At least 30 cellular or viral
protein factors have been found to physically interact with YY1 protein, and over 70 gene promoters have been found to be regulated by YY1.
cDNAs encoding YY1 or YY1 homologs have been cloned from humans, rodents, zebrafish, frogs, fruitfly, and maize. Human and mouse YY1
protein sequences are highly homologous; regions of near 100% sequence homology include the acidic domain, glycine-rich domain, histidine-rich
domain, and the zinc finger domain. YY1 is usually expressed at higher levels in proliferating cells. Expression of YY1 is typically low in post-mitotic
tissues, and can be down-regulated during cell differentiation. The consensus promoter DNA sequence recognized by YY1 is
(C/g/a)(G/t)(G/t/a)CATN(T/a)(T/g/c). Although human YY1 has a calculated molecular weight of ~45 kDa, it exhibits a prominently retarded gel
mobility corresponding to ~70 kDa in SDS-PAGE.
Biomedical Research Service
& Clinical Application
A 32P-labeled DNA fragment containing
the skeletal alpha-actin promoter was
incubated with 10 ng of purified YY1, and
the YY1-DNA complex was resolved by a
5% polyacrylamide gel.