Urea Assay Kit                                                                                                                                                                                                  Price

Product Description: Urea is the major organic component of human urine, representing the end waste product of protein metabolism. Amino
acids are metabolized and converted to ammonia, which at high levels is toxic to cells. The urea cycle, which occurs in the liver, mediates the
removal of ammonia as urea in the amount of 10 to 20 g per day. A defective urea cycle may therefore cause hyperammonemic encephalopathy
and brain injury. A failure of ureagenesis can occur due to acquired disease, e.g., cirrhosis secondary to alcoholism, or secondary to a congenital
enzymopathy. Since urea is carried by blood to the kidneys for elimination from the body, high levels of urea in the blood may indicate renal
failure. Elevated blood urea may also be caused by use of corticosteroids, dehydration, or congestive heart failure. Decreased blood urea levels
can occur in response to liver disease, malnutrition, or over-vigorous parenteral fluid therapy. Urea determination is thus useful for assessing the
kidney function of patients. The Urea Assay Kit is non-enzymatic, and is based on the modified Jung method (Biochem Cell Biol. 87:541–544,
2009), which involves a specific condensation reaction of o-phthalaldehyde with urea. The formed carbonium ion further reacts with a coloring
compound, thus enabling measurement of urea by light absorbance at 430 nm. Note that the assay solution contains 2.5M sulfuric acid and should
be handled with caution.

Kit Components:

Urea Assay Solution-I: 5 ml (for 200 wells), store at 4ºC
Urea Assay Solution-II: 5 ml, store at 4ºC
Urea Assay Solution-I
II: 0.15 ml, store at 4ºC
Urea Standard:
1 g, store at room temperature

MSDS: boric acid, o-phthalaldehyde, primaquine bisphosphate, sulfuric acid

Related kits: ALP Assay, TRAP Assay, Serum Albumin Assay
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