Tartrate-resistant Acid Phosphatase (TRAP) Assay Kit                                                                                                                              Price

Product Description: Tartrate-resistant acid phosphatase (TRAP), encoded by the ACP5 gene, is highly conserved throughout mammalian
cells, playing a significant role in processes such as bone mineralization, ECM remodeling, cytokine production, and immune regulation. Enzyme
catalysis is mediated through the binding of a phosphate substrate to the Fe2+ in the active site of TRAP. Bone metabolism is coordinated
between osteoblast-mediated bone formation and osteoclast-mediated bone resorption. Since TRAP is expressed by osteoclasts, the enzyme
and alkaline phosphatase (ALP) are used as markers of osteoclasts and osteoblasts, respectively, and their serum levels serve as a diagnostic
for relevant bone diseases. In addition, TRAP expression can be increased in several other pathological conditions, e.g., hairy cell leukemia, HIV-
induced encephalopathy and Gaucher’s disease. The assay kit is based on conversion of p-nitrophenol phosphate to nitrophenol in an acidic
buffer. Nitrophenol exhibits an absorption maximum at 405 nm (molar extinction coefficient = 18.75 mM-1cm-1), allowing for sensitive detection of
TRAP activity in crude samples. Assay solution is stable for several years if stored and handled properly.

Kit Components:

10x Cell Lysis Solution: 25 ml, store at 4ºC
TRAP Assay Solution: 20 ml, store in aliquots at -20ºC (200 assays)

MSDS: TX-100, nitrophenol phosphate, Acetic acid, Sodium Tartrate

Related kits: beta-Galactosidase (LacZ) Assay, Alkaline Phosphatase Assay, Luciferase Assay, Osteogenesis Assay, Chondrogenesis Assay
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