TMB Substrate Kit (for ELISA application) Price
Product Description: One of the most widely used enzymes for chromogenic detection in immunoassays is horseradish peroxidase (HRP), a
highly stable enzyme (Clin. Chim. Acta 81:1,1977). The compound 3,3',5,5'-tetramethylbenzidine (TMB) has emerged as the safest colorigenic
substrate of HRP exhibiting excellent sensitivity (Tetrahedron 30:3299,1974). HRP in the presence of peroxide converts TMB to a bluish reaction
product that can be read spectrophotometrically at 370 or 655 nm. The blue end product upon acidification by HCl or H2SO4 generates a bright
yellowish color enabling sensitive HRP detection and quantification by absorbance at 450 nm. This application has become widely used in
enzyme-linked immunosorbent assay (ELISA) because of its lowest detection limit compared to other reporter enzymes and substrate systems.
The working substrate solution needs to be prepared fresh by mixing TMB Substrate solution and Peroxide solution. The ELISA Detection kit is
stable for ~1 year if stored and handled properly.
TMB Substrate: 16 ml, store at room temperature
Stabilized Peroxide: 200 ml, store at room temperature
MSDS: TMB, Methanol, Hydrogen peroxide, Hydrochloric acid
Related kits: DAB Substrate kit, BCIP Substrate kit, Protein Assay Kit (EDTA/Mg2+ compatible), Protein Assay Kit/D (detergent compatible)
Mastri et al
Activation of Toll-like receptor 3 amplifies mesenchymal stem cell trophic factors and enhances therapeutic potency.
Am J Physiology 303:C1021-1033, 2012
Biomedical Research Service
& Clinical Application
Culture media were analyzed by TNF-alpha
ELISA using an HRP-conjugated
secondary antibody. The ELISA Detection
TMB substrate solution was used for
quantification. The study shows that IL-6
caused downregulation of TNF-alpha in
cultured myoblasts, demonstrating an
anti-inflammatory property of IL-6.