Threonine Dehydrogenase (TDH) Assay Kit                                                                                                                                                  Price

Product Description: Threonine is an essential amino acid with critical nutritional and metabolic function. Two major pathways for the
degradation of L-threonine in mammals are those mediated by threonine dehydratase and threonine dehydrogenase (TDH). While the former
enzyme converts threonine to ammonium and 2-ketobutyrate,  which is eventually oxidized to CO2, TDH forms 2-amino-3-ketobutyrate.
2-amino-3-ketobutyrate is further cleaved to form glycine and acetyl-CoA, or undergoes spontaneous decarboxylation yielding aminoacetone and
CO2. TDH is a member of the zinc-containing long-chain alcohol/polyol dehydrogenase family. Threonine catabolism mediated by TDH has
recently been recognized as a specific metabolic trait of mouse embryonic stem cells, possessing a potential regulatory role in stem cell
programming. Our non-radioactive colorimetric TDH assay is based on the reduction of the tetrazolium salt INT in a NADH-coupled enzymatic
reaction to formazan, which is water-soluble and exhibits an absorption maximum at 492 nm. The intensity of the red color formed is increased in
the presence of increased TDH activity. The kit is stable for at least one year under proper storage and handling conditions.

Kit Components:

TDH Assay Solution: 10 ml, store at -80ºC (for 200 wells)
10x TDH Substrate: 1 ml, store at -80ºC
10x Cell Lysis Solution: 25 ml, store at 4ºC

MSDS: ethanol, TX-100, DMSO, INT, acetic acid, Hepes

Related kits: ADH Assay, GAPDH Assay, SDH Assay
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