Pyruvate Dehydrogenase (PDH) Assay Kit                                                                                                                                                    Price

Product Description: Pyruvate is the end product of aerobic glycolysis and is at an important metabolic crossroad. The pyruvate
dehydrogenase multi-enzyme complex (PDH) catalyzes the oxidative decarboxylation of pyruvate to acetyl-CoA in a series of reactions mediated
by the E1 subunit (pyruvate dehydrogenase), E2 subunit (dihydrolipoyl transacetylase), and E3 subunit (dihydrolipoyl dehydrogenase). The
reactions of the PDH complex serve to interconnect the metabolic pathways of glycolysis, gluconeogenesis and fatty acid synthesis to the Krebs
(TCA) cycle. As a consequence, the PDH complex is highly regulated by a variety of allosteric effectors and by covalent modification. The
importance of the PDH complex in metabolic regulation is evident from the fact that although diseases associated with deficiencies of the PDH
complex have been observed, affected individuals often do not survive to maturity. This non-radioactive colorimetric PDH assay is based on the
reduction of the tetrazolium salt INT in a NADH-coupled enzymatic reaction to formazan, which is water-soluble and exhibits an absorption
maximum at 492 nm. The intensity of the red color formed is increased in the presence of increased PDH activity. The kit is stable for at least one
year under proper storage and handling conditions.

Kit Components:

PDH Assay Solution: 5 ml, store at -80ºC
10x Cell Lysis Solution: 25 ml, store at 4ºC

MSDS: TX-100, DMSO, INT, acetic acid, Hepes

Related kits: HK Enzyme Assay, GAPDH Enzyme Assay, LDH Enzyme Assay, L-Lactate Assay, Glucose Assay, Pyruvate Assay, NAD+ Assay

Citation:
Missihoun et al
Myocardial oxidative stress, osteogenic phenotype, and energy metabolism are differentially involved in the initiation and early progression of
delta-sarcoglycan-null cardiomyopathy.
Molecular and Cellular Biochemistry 321:45-52, 2009

Martin et al
Androgen deprivation leads to increased carbohydrate metabolism and hexokinase 2-mediated survival in Pten/Tp53-deficient prostate cancer
Oncogene 36(4):525-533, 2017
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Biomedical Research Service
& Clinical Application
PDH assays were performed using heart
extracts prepared from normal (F1B
strain) and cardiomyopathic (TO2 strain)
hamsters at 1 and 4 months.  Pelleted
membrane fractions were used for PDH
activity assay. The study shows that the
cardiomyopathic hamster heart fails to
exhibit an age-dependent increase in
PDH activity.
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