Nagalase Assay Kit                                                                                                                                                                                         Price

Product Description: The human lysosomal enzyme α-NAGAL (or Nagalase; E.C. 3.2.1.49) removes O-linked α-GalNAc from glycolipids and
glycoproteins. In lysosomal storage disease (LSD), enzyme deficiency in a patient leads to the accumulation of substrate in the tissues, resulting
in the development of clinical symptoms. Schindler/Kanzaki disease, a rare LSD caused by deficient Nagalase, displays several phenotypes from
severe infantile neurodegeneration to mild cognitive impairments. Individuals with defective Nagalase can abnormally process their blood group
antigens. Notably, recent cancer research has suggested that Nagalase facilitates the deglycosylation of Macrophages Activator Factor (Gc-
MAF), which in turn inhibits the activation of macrophages. Since macrophages play a vital role in the fight against cancer cells, measurement of
Nagalase activity bears clinical significance. The Nagalase assay is based on the enzymatic cleavage of the artificial substrate p-nitrophenyl-
alpha-N-acetyl-galactosaminide to nitrophenol in an acidic buffer (Marine Drugs 16, 155, 2018). Ionization of nitrophenol by NaOH produces a
yellow color exhibiting an absorption maximum at 405-415 nm, which allows for sensitive detection of Nagalase activity in crude cell/tissue
extracts, serum/plasma and urine. The assay is designed for the 96-well plate format. Repeated freeze-thaw cycles of the assay solution should
be avoided.

Kit Components:

10x Cell Lysis Solution: 25 ml, store at 4ºC
Nagalase Assay Solution: 5 ml (100 wells), store in aliquots at -20ºC
Nagalase Control Solution: 5 ml, store at 4ºC

MSDS: p-nitrophenyl-a-N-acetyl-galactosaminide, TX-100, sodium acetate, acetic acid

Related kits: OGA Assay
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Left: Mice were fed methionine- and choline-deficient
(MCD) diet for 6 weeks. Liver tissues were homogenized
and Nagalase enzyme activities were determined by the
Nagalase Assay kit, showing that the fatty liver exhibits a
higher level of Nagalase enzyme activities. Right:
Hamster muscle tissues were collected from the normal
strain (F1B) and dystrophic strain (TO2). Muscle
Nagalase activities were assayed, showing that muscular
dystrophy is associated with a higher level of Nagalase
enzyme activity.