NAD+ Assay Kit                                                                                                                                                                                               Price

Product Description: In metabolism, the compound nicotinamide adenine dinucleotide accepts or donates electrons in redox reactions, forming
the NAD+/NADH pair with a redox potential of -0.32 volts. The NAD+/NADH ratio is an important measurement that reflects both the metabolic
and health states of cells. Key enzymes regulating the NAD+/NADH ratio are those dehydrogenases involved in fuel metabolism (such as lactate
dehydrogenase and pyruvate dehydrogenase) and electron transport chain complexes (such as NADH dehydrogenase). Non-redox roles of
NAD+ have received increasing attention in recent years. For instance, NAD+ is consumed in ADP-ribosylation reactions mediated by ADP-
ribosyltransferase and poly(ADP-ribose) polymerase. Sirtuins, a group of NAD-dependent deacetylases, also consume NAD+, and this
enzymatic activity appears to be critically involved in the aging of vertebrates and invertebrates. The NAD+ Assay Kit is based on LDH-mediated
reduction of NAD+ and the tetrazolium salt INT in a diaphorase-coupled reaction, which generates a formazan product that can be measured by
absorbance at 492 nm. As low as 1 − 2 uM NAD+ may be detected by the assay. Assay reagents are stable for at least 1 year if stored and
handled properly.

Kit Components:

NAD+ Assay Solution: 5 ml; store at -80°C (100 assays)
4 mM NAD+ Standard: 0.1 ml; store at -80°C

MSDS: DMSO, INT, acetic acid, Hepes

Related kits: LDH Assay, PDH Assay, L-Lactate Assay, ATP Assay, Pyruvate Assay
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