Mini-Plasmid DNA Purification Kit                                                                                                                                                                               Price

Product Description: Isolation of high-quality plasmid DNA from the gemisch of the E. coli cell lysate paves the way for many laboratory as well as clinical
genetic applications, among which are clone screening, DNA sequencing, DNA transfection, probe preparation, PCR analysis, and gene therapy, to name
but a few. Small-scale plasmid purification (mini-prep) is often used to facilitate clone screening and DNA sequencing. Although many commercial mini-
prep kits are offered, few combines the following three features: simplicity, low cost, and high quality. We have modified and streamlined the alkaline lysis
mini-prep method of Sambrook et al. (Molecular Cloning, CSH, NY) eliminating the needs for additional RNase treatment and phenol-chloroform extraction.
Plasmid DNA can be easily and rapidly purified from an overnight E. coli culture in 15 min with a typical yield of 1 – 5 ug DNA per ml of culture. The DNA is
suitable for restriction digestion, probe preparation, and PCR analysis. DNA to be used for sequencing analysis is subjected to an additional 10-min step
of PEG precipitation (PS-4) to remove any trace amount of RNA. High-quality DNA sequencing ladders can be easily obtained (almost 1,000 bases
resolved per sequencing run) if the plasmid DNA is isolated from a suitable E. coli host strain. The kit (sufficient for 300 mini-preps) is stable for at least
one year if handled and stored properly.

Kit Components:

Plasmid Solution-1 (PS-1): 30 ml, store at 4ºC (sufficient for 300 mini-preps)
Plasmid Solution-2 (PS-2): 30 ml, store at room temperature
Plasmid Solution-3 (PS-3): 30 ml, store at 4ºC
Plasmid Solution-4 (PS-4): 6 ml, store at 4ºC

MSDS: Tris, EDTA, SDS, NaOH, RNase A, PEG-800, potasium acetate, acetic acid, ethanol, isopropanol

Related kits: Genomic DNA Isolation, Whole Blood DNA Isolation, DNA Transfection, Maxi-Plasmid DNA Purification, E coli Competent Cell Preparation Kit

Citation:
Lin et al
Role and differential expression of calpastatin mRNA and protein in cultured cardiomyocytes exposed to hypoxic stress
Mol Cell Biochemistry 265:63-70, 2004
Site Map
Biomedical Research Service            
& Clinical Application
Plasmid DNA was isolated using our simple and
inexpensive Plasmid DNA Purification kit. Each
sequencing run can generate up to ~1kb sequence
information.
Alphabetical Listing