Maxi-Plasmid DNA Purification Kit                                                                                                                                                                 Price

Product Description: Isolation of high-quality plasmid DNA from the gemisch of the E. coli cell lysate paves the way for many laboratory as well
as clinical genetic applications, among which are clone screening, DNA sequencing, DNA transfection, probe preparation, PCR analysis, and
gene therapy, to name but a few. Although small-scale plasmid purification (mini-prep) is often sufficient for DNA cloning and sequencing, large-
scale (maxi-prep) plasmid purification is required for gene transfer and gene therapy studies. Our Maxi-Plasmid Isolation Kit is similar to the Mini-
Plasmid Isolation Kit with additional filtration and precipitation steps. Plasmid DNA can be easily and rapidly purified without the use of phenol.
Each Maxi-prep DNA is purified from 100 - 200 ml of overnight E. coli culture in 90 min with a typical yield of 0.5 – 3 ug DNA (as determined by
plasmid replication origin) per ml of culture. Purified plasmid DNA, typically with an A260/A280 ratio of ~2, is suitable for restriction digestion,
probe preparation, DNA sequencing, PCR analysis, and transfection. The kit (sufficient for 10 maxi-preps) is stable for at least one year if
handled and stored properly.

Kit Components:

Plasmid Solution-1 (PS-1): 40 ml, store at 4ºC
Plasmid Solution-2 (PS-2): 40 ml, store at room temperature
Plasmid Solution-3 (PS-3): 40 ml, store at 4ºC
Plasmid Solution-4 (PS-4): 6 ml, store at 4ºC
Precipitation Buffer: 10 ml, store at 4ºC
Filter Disc: 10 units

MSDS: Tris, EDTA, SDS, NaOH, RNase A, PEG-800, potasium acetate, acetic acid, ethanol, isopropanol

Related kits: Genomic DNA Isolation, Whole Blood DNA Isolation, DNA Transfection, Mini-Plasmid DNA Purification, E coli Competent Cell
Preparation Kit

Citation:
Lin et al
Role and differential expression of calpastatin mRNA and protein in cultured cardiomyocytes exposed to hypoxic stress
Mol Cell Biochemistry 265:63-70, 2004
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Plasmid DNA was isolated using our simple and
inexpensive Plasmid DNA Purification kit. Each
sequencing run can generate up to ~1kb sequence
information.
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