MMP2/MMP9 Solution Assay (SA) Kit                                                                                                                                                             Price

Product Description: Matrix metalloproteinases (MMPs) are secreted or transmembrane endopeptidases belonging to the larger protease
family known as the metzincin superfamily. Although MMPs were first found in vertebrates in 1962, they have since been found in invertebrates
and plants. All MMPs are synthesized as zymogens requiring extracellular activation, which can be activated in vitro by organomercurials,
denaturing agents, and proteases. MMPs control extracellular matrix (ECM) remodeling through their matrix-degrading activities, which play
critical roles in many normal growth and developmental processes. Dysregulation of MMP activity has thus been implicated in many pathological
conditions. MMP2 (Gelatinase A, 72 kDa) and MMP9 (Gelatinase B, 92 kDa) are thought to be particularly important in cancer metastasis. The
MMP2/MMP9 Solution Assay kit is based on MMP2/MMP9-mediated hydrolysis of blue gelatin, which allows for convenient and sensitive analysis
of MMP activity. In this assay format, MMP activity is dictated by the balance between MMPs and their endogenous inhibitors (TIMPs). In addition,
the assay system is compatible with large-scale screening of compounds capable of MMP inhibition. MMP activity is determined by optical
measurement of 595nm after acetone precipitation of undigested blue gelatin. We also offer a complementary
MMP2/MMP9 Gel Assay (GA)
Kit
, which is based on gelatin zymography. The gel assay format is more tedious, but can discriminate between MMP2 and MMP9.

Kit Components:

5x MMP Buffer: 1 ml; store refrigerated
Blue Gelatin: 1 ml (100 reactions); store refrigerated

MSDS: Tris, Acetone (Not included in the kit)

Related kits: MMP2/MMP9 Gel Assay (GA) Kit, Protein Assay Kit/DC
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