MMP2/MMP9 Gel Assay (GA) Kit                                                                                                                                                                  Price

Product Description: Matrix metalloproteinases (MMPs) are secreted or transmembrane endopeptidases belonging to the larger protease
family known as the metzincin superfamily. Although MMPs were first found in vertebrates in 1962, they have since been found in invertebrates
and plants. All MMPs are synthesized as zymogens requiring extracellular activation, which can be activated in vitro by organomercurials,
denaturing agents, and proteases. MMPs control extracellular matrix (ECM) remodeling through their matrix-degrading activities, which play
critical roles in many normal growth and developmental processes. Dysregulation of MMP activity has thus been implicated in many pathological
conditions. MMP2 (Gelatinase A, ~70 kDa for latent form) and MMP9 (Gelatinase B, ~90 kDa for latent form) are thought to be particularly
important in cancer metastasis. The MMP2/MMP9 assay kit is based on a zymography method for visualizing the gelatin hydrolytic activities of
MMP2 and MMP9 present in tissue extracts, cell cultures, serum or urine. After electrophoresis the gel is incubated overnight for MMP activation,
during which time gelatin in the region of an active MMP band is digested to fragments sufficiently small to diffuse out of the gel. The gel gives
rise to a clear band in the dark blue background upon staining in Coomassie brilliant blue. We also offer a complementary
MMP2/MMP9
Solution Assay (SA) Kit, which can be used for rapid screening of compounds capable of inhibiting MMP. Please note that the kit does NOT
include the glass plates, spacers and reagents for gel casting. Customers also need to prepare a Coomassie Brilliant Blue solution for gel
staining.

Kit Components:

5x MMP Reaction Buffer: 180 ml; store at room temperature
5x SDS-PAGE Loading Buffer: 5 ml; store at room temperature
50x CaCl
2 Solution: 20 ml; store at room temperature
1000x ZnSO
4 Solution: 1 ml; store at room temperature
TX-100: 15 ml; store at room temperature
Gelatin: 0.15 g; store at room temperature

Technical document: Handcasting polyacrylamide gel

MSDS: TX-100, SDS, Tris, TEMED, Ammonium Persulfate, acrylamide (TEMED, Ammonium persulfate and acrylamide not included in the kit)

Related kits: Protein Assay Kit/DC, MMP2/MMP9 Solution Assay (SA) Kit

Citation:
Mastri et al
Secreted Frizzled-related protein 2 as a target in antifibrotic therapeutic intervention
Am J Physiol Cell Physiol, 306:C531, 2014
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