L-Lactate Assay Kit                                                                                                                                                                                         Price

Product Description: Production of L-lactate mediated by the enzyme lactate dehydrogenase (LDH) is increased under hypoxic or anaerobic
conditions. Measurement of cellular lactate concentration is thus of interest in the study of cellular as well as animal physiology, providing an
important index of the balance between tissue oxygen demand and utilization (see assay on right). Our Lactate Assay Kit is based on the
reduction of the tetrazolium salt INT in a NADH-coupled enzymatic reaction to formazan, which is water-soluble and exhibits an absorption
maximum at 492 nm. Since the intensity of the red color formed is proportional to the lactate concentration, the assay, using a set of lactate
standards, can measure the concentration of lactate in the cell and released to the culture medium, circulation, or urine in a quantitative manner.
The non-radioactive assay kit allows the assay to be completed in one step within 30-60 min. The kit can be used for clinical samples such as
serum, plasma, and urine. The assay solution is stable for several years if stored and handled properly.

Kit Components:

L-Lactate Assay Solution: 10 ml (200 wells); store at -80°C shielded from light
1 mM L-Lactate Standard: 0.5 ml; store at -20°C
PEG-8000 Solution: 5 ml; store at 4°C

MSDS: DMSO, INT, acetic acid, Tris, PEG

Related kits: D-Lactate Assay, LDH Assay, PDH Assay, ATP Assay, Pyruvate Assay, NAD+ Assay

Gordon et al
Brain metabolism dictates the polarity of astrocyte control over arterioles
Nature, 456, 745-749, 2008

Martin et al
Tissue-nonspecific alkaline phosphatase as a target of sFRP2 in cardiac fibroblasts
Am J Physiol Cell Physiol, 309:C139, 2015

Fernandez et al
Effects of dairy products on intestinal integrity in heat-stressed pigs
Temperature 1:128-134, 2014
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Biomedical Research Service
& Clinical Application
HEK293 cells constitutively expressing bcl-2
show altered glucose metabolism. Culture
media were diluted 10 fold and mixed with the
L-Lactate Assay Solution. L-lactate
concentrations were deduced from a lactate
standard plot. The assay reveals that lactate
production by the Bcl-2 expressor is reduced
compared to the parental HEK293 cells.
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