Lactate Dehydrogenase (LDH) Staining Kit                                                                                                                                                    Price

Product Description: Lactate dehydrogenase (LDH) is an oxidoreductase which catalyzes the interconversion of lactate and pyruvate. It consists
of 4 subunits which may be of 2 different types: M (muscle) and H (heart), also known as A and B respectively. Five different isoenzymes are
therefore possible, depending on the subunit composition. The LDH staining kit is based on the reduction of a tetrazolium salt to a formazan
product, which is water insoluble and exhibits an intense dark blue color. Since the intensity of the dark blue color is proportional to LDH activity,
the staining kit can be used to delineate cell/tissue LDH isoform activity by either gel assay or in situ staining (see gel on right).

Kit Components:

LDH Staining Solution: 40 ml, store at -80°C
10x Cell Lysis Solution: 25 ml, store at 4ºC
Loading Solution: 0.5 ml, store at 4ºC

MSDS: TX-100, MTT, Hepes, DMSO

Related kits: HK Enzyme Assay, GAPDH Enzyme Assay, LDH Enzyme Assay, Glucose Assay, D-Lactate Assay, PDH Enzyme Assay, G6PD
Enzyme Assay, Pyruvate Assay, NAD+ assay, L-Lactate Assay
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Mouse heart tissue extracts (10 ug)
were fractionated by a 0.8%
non-denaturing agarose gel,
following which the LDH staining
solution was applied to the gel and
incubated at 37C for 1 hour.
Reduction of tetrazolium revealed
the five dark blue LDH bands. Note
that LDH-5 barely migrated through
the well.  
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