Inosine-5′-monophosphate Dehydrogenase (IMPDH) Assay Kit                                                                                                          Price

Product Description: Inosine-5′-monophosphate dehydrogenase (IMPDH) is a purine metabolic enzyme catalyzing the first and rate-limiting
step towards the synthesis of guanosine triphosphate (GTP). IMPDH converts inosine monophosphate (IMP) to xanthosine monophosphate
(XMP) using NAD+ as a cofactor, providing precursors for DNA and RNA bioysynthesis. The protein has an additional role as a DNA-binding
transcriptional repressor. Since IMPDHs are known to exhibit species-specific kinetic properties and inhibitor sensitivities, the enzymes have
been the target of antiviral, antibacterial and anticancer therapeutics. The measurement of IMPDH activity thus serves as a useful tool for drug
design and screening. Indeed, several pharmaceutical companies have used the kit for their drug screening application. The IMPDH assay is
based on the reduction of the tetrazolium salt INT in a NADH-coupled enzymatic reaction to INT-formazan. The intensity of the red color formed
is increased in the presence of increased activity
of IMPDH1 or IMPDH2. The assay solution should be stored in aliquots at -80ºC.

Kit Components:

IMPDH Assay Solution: 5 ml, store at -80°C (for 100 wells)
50x IMPDH Substrate: 0.1 ml, store at -80ºC                                 
10x Cell Lysis Solution: 25 ml, store at 4ºC

MSDS: TX-100, DMSO, INT, acetic acid, Hepes

Related kits: HK Enzyme Assay, G6PD Enzyme Assay, Adenylate Kinase Assay
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HeLa cells were treated with
DMSO (control) and 10 uM
mycophenolic acid (MPA) for 24
hours, following which cells were
harvested and IMPDH activity was
measured using the IMPDH assay
Purified human IMPDH1 enzyme was
incubated with a novel compound at the
concentration indicated. Enzyme activity
was assayed by the IMPDH Assay kit. The
assay is used to determine the IC
50 of the
novel compound against IMPDH1.