L-Glutamine/Glutamate Assay Kit Price
Product Description: Glutamine is a glucogenic amino acid and the most abundant free amino acid in human blood. The amino acid is
synthesized by glutamine synthetase from glutamate and ammonia predominantly in muscle, accounting for ~90% of all glutamine synthesized.
Supplementation with glutamine is therefore beneficial during times of skeletal muscle wasting. Although normally synthesized in adequate
amounts, endogenous glutamine production may be inadequate during periods of metabolic stress. In the anaplerotic pathway, which can be
upregulated in some cancer cells, glutamine can be converted via glutaminase and glutamate dehydrogenase to α-ketoglutarate and
incorporated into the Krebs cycle where it can serve as a supplementary carbon. In addition, glutamine transports NH3 to the kidney to maintain
acid-base balance and between organs for biosynthesis of nucleotides, amino sugars, and glutathione. The assay is based on (1) conversion of
glutamine to glutamate by glutaminase (see accompanied graph below), and (2) glutamate oxidation/deamination by glutamate dehydrogenase
in the presence of NAD+. The NADH formed can be measured colorimetrically at 492 nm using a microplate format. Kit components are stable
for at least one year if stored and handled properly.
Glutamine Conversion Buffer: 20 ml, store at 4ºC
Glutaminase: 25 ul, store at 4ºC (gently vortex vial to disperse enzyme precipitates prior to pipetting)
Glutamate Assay Solution: 5 ml (for 100 wells), store at -70ºC (avoid light exposure)
10 mM L-Glutamate: 0.2 ml, store at -20ºC or -70ºC
PEG Solution: 5 ml; store at 4°C (viscous; pipette solution with a cut tip)
MSDS: Tris, sodium acetate, acetic acid, DMSO, INT, PEG
Related kits: L-Lactate Assay, ATP Assay, NAD+ Assay, alpha-KG Assay, Glutamate Assay, Pyruvate Assay
Biomedical Research Service
& Clinical Application
Conversion of glutamine to glutamate
in step 1 of the assay.