L-Glutamate Assay Kit                                                                                                                                                                                       Price

Product Description: The non-essential amino acid glutamate is a key molecule in cellular metabolism, serving as an intermediate in
transamination and deamination reactions, which allows nitrogen from amino acids to be excreted. Glutamate is also a critical neurotransmitter
involved in learning and memory. In brain injury however, glutamate accumulates outside cells, causing excitotoxicity. In the food industry, glutamate
is widely used as a flavor enhancer. The side chain carboxylic group has a pKa of 4.1, thus conferring a negative charge at physiological pH. Our
non-radioactive colorimetric glutamate assay is based on the reduction of the tetrazolium salt INT in a NADH-coupled enzymatic reaction to
formazan, which is water-soluble and exhibits an absorption maximum at 492 nm (detection limit ~20 uM). The intensity of the red color formed is
increased in the presence of increased L-glutamate. Please note that tissue, plasma, and serum samples need to be deproteinized prior to assay.
The Assay Solution is stable for several years if stored and handled properly.

Kit Components:

L-Glutamate Assay Solution: 10 ml (for 200 wells), store at -70ºC (avoid light exposure)
10 mM L-Glutamate: 0.2 ml, store at -20ºC or -70ºC
PEG-8000 Solution: 5 ml; store at 4°C (viscous, pipette solution with a cut tip)

MSDS: DMSO, INT, acetic acid, Tris

Related kits: Glutamate Dehydrogenase (GLDH) Assay, Pyruvate Assay, alpha-KG Assay, Glutamine Assay
Biomedical Research Service
& Clinical Application
Heart tissues from normal hamster (F1B
strain) and cardiomyopathic hamster
(TO2 strain) were extracted with TCA and
analyzed by the L-Glutamate assay. The
study shows that the cardiomyopathic
heart contains an increased level of
L-glutamate due to chronic myocyte
degeneration.
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