L-Glutamate Assay Kit                                                                                                                                                                                       Price

Product Description: The non-essential amino acid glutamate is a key molecule in cellular metabolism, serving as an intermediate in
transamination and deamination reactions, which allows nitrogen from amino acids to be excreted. Glutamate is also a critical neurotransmitter
involved in learning and memory. In brain injury however, glutamate accumulates outside cells, causing excitotoxicity. In the food industry, glutamate
is widely used as a flavor enhancer. The side chain carboxylic group has a pKa of 4.1, thus conferring a negative charge at physiological pH. Our
non-radioactive colorimetric glutamate assay is based on the reduction of the tetrazolium salt INT in a NADH-coupled enzymatic reaction to
formazan, which is water-soluble and exhibits an absorption maximum at 492 nm. The intensity of the red color formed is increased in the presence
of increased L-glutamate. Please note that tissue, plasma, and serum samples need to be deproteinized prior to assay. Each kit is sufficient for 200
microassays. The Assay Solution should be stored at -80ºC, and will remain stable indefinitely at -80ºC.

Kit Components:

L-Glutamate Assay Solution: 10 ml (200 wells), store at -80ºC shielded from light
8 mM L-Glutamate: 1 ml, store at -20ºC or -80ºC
PEG-8000 Solution: 5 ml; store at 4°C

MSDS: DMSO, INT, acetic acid, Hepes

Related kits: Glutamate Dehydrogenase (GLDH) Assay Kit
Biomedical Research Service
& Clinical Application
Heart tissues from normal hamster (F1B
strain) and cardiomyopathic hamster (TO2
strain) were extracted with TCA and
analyzed by the L-Glutamate assay. The
study shows that the cardiomyopathic heart
contains an increased level of L-glutamate
due to chronic myocyte degeneration.
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