Gel Shift Assay Kit                                                                                                                                                                                             Price

Product Description: Gel shift assay, also known as gel retardation assay or electrophoretic mobility shift assay (EMSA), is widely used for the
detection of DNA/RNA-protein complexes. Gel shift assays performed with total or nuclear protein extracts require the use of nonspecific DNA
competitors, usually polyanion polymers, to sequester non-specific DNA-protein interaction. The choice of nonspecific competitors however can be
tricky since DNA-binding proteins can exhibit different intrinsic binding modes, specificities and affinities, which may be inadvertently attenuated by
a nonspecific competitor (Nucl. Acids Res. 20:140,1992; BioTechniques 15:390,1993). In addition, salt concentrations in the binding buffer may
differentially affect DNA-protein and protein-protein interactions (Mol. Cell. Biol. 11:5090,1991). These factors, if not properly identified and
controlled, may lead to irreproducible results. Using a set of dissimilar polyanion competitors and different binding conditions may thus facilitate the
detection of functionally relevant DNA-binding activities. Our Gel Shift Assay Kit includes a set of three different polyanion polymers: poly(dI-dC),
poly R-478, and sheared salmon sperm DNA. The binding assay can be performed in two different salt concentrations (10 and 100 mM KCl).
Components of the assay kit are stable for at least one year if stored and handled properly.

Kit Components:

10x GS Buffer (low): 1 ml, store at 4ºC
10x GS Buffer (high): 1 ml, store at 4ºC
1 mg/ml poly(dI-dC): 0.1 ml, store at 4ºC
1 mg/ml poly R-478: 0.1 ml, store at 4ºC
1 mg/ml Salmon DNA: 0.1 ml, store at 4ºC
GS Loading Solution: 1 ml, store at 4ºC

MSDS: Tris, EDTA, bromophenol blue, DTT, poly R-478

Related kits: Genomic DNA Isolation, Whole Blood DNA Isolation, DNA Transfection, Maxi-Plasmid DNA Purification

Citation:
Lee & Schwartz
Novel use of a polymeric chromophore in detecting sequence-specific DNA-binding proteins
BioTechniques 15:390, 1993
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& Clinical Application
Myoblasts were transfected with 20 and 500
ng of pMSV-SRF plasmid DNA. Nuclear
extracts were prepared from the cells and
analyzed by the gel shift assay using a
P32-labeled alpha-actin promoter DNA as
probe. The gel shift assay identified the SRF-
and YY1-containing DNA complexes
simultaneously.
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