Glutathione S-transferase (GST)                                                                                                                                                                     Price

BACKGROUND: Many recombinant proteins are expressed as GST-fusion proteins to facilitate purification. The recombinant GST protein is
encoded by the parasitic Schistosoma japonicum glutathione S-transferase gene. The parasite GST protein shows ~60% homogy with class Mu
isozymes of mammalian GST (EC 2.5.1.18). The mammalian GST super family consists of four classes: Alpha, Mu, Pi, and Theta. GST catalyzes
the conjugation of reduced glutathione to electrophiles on many substrates, thus detoxifying endogenous compounds such as lipid peroxides. The
recombinant GST can be used as a negative control for recombinant growth factors expressed as GST fusion proteins. The C-terminal end of the
protein harbors an engineered thrombin cleavage site and a protein kinase A (PKA) phosphorylation site. The protein can therefore be
radioactively labeled with 32P-g-ATP and PKA.
Site Map
One ug of purified GST
protein was analyzed by
10% SDS-PAGE.
Biomedical Research Service
& Clinical Application
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