Glycerol-3-Phosphate Dehydrogenase (GPDH) Assay Kit                                                                                                          Price

Product Description: Glycerol-3-phosphate dehydrogenase (GPDH) catalyzes the reversible conversion of dihydroxyacetone phosphate
(DHAP) to glycerol-3-phosphate (G3P). In the glycerol phosphate shuttle, DHAP is reduced by the cytosolic GPDH (GPDH1 or cGPDH), which is
NADH dependent, to G3P. G3P can diffuse from the cytoplasm to the inner mitochondrial membrane where it is oxidized back to DHAP by the
mitochondrial GPDH (GPDH2 or mGPDH), which is FAD-dependent. The cytosolic and mitochondrial GPDH enzymes thus constitute an electron
shuttle mechanism that facilitates the transfer of reducing equivalents from the cytosol to mitochondria. Since G3P also plays a role in lipid
biosynthesis, GPDH serves to coordinate carbohydrate and lipid metabolism. GPDH activity is also known to increase during adipogenic
differentiation. The enzyme activity assay kit can be used to quickly assess potential changes in the expression and regulation of GPDH1. The
non-radioactive colorimetric GPDH assay is based on the reduction of the tetrazolium salt INT in a NADH-coupled enzymatic reaction to
formazan, which is water-soluble and exhibits an absorption maximum at 492 nm. The intensity of the red color formed is increased in the
presence of increased GPDH activity. The kit is stable for at least one year if handled and stored properly.

Kit Components:

GPDH Assay Solution: 5 ml, store at -80°C (for 100 wells)      
50x GPDH Substrate: 0.1 ml, store at -80ºC                           
10x Cell Lysis Solution: 25 ml, store at 4ºC

MSDS: TX-100, DMSO, INT, acetic acid, Hepes

Related kits: HK Enzyme Assay, Glucose Assay, LDH Enzyme Assay, PDH Enzyme Assay, G6PD Enzyme Assay, GAPDH Assay
Site Map
Biomedical Research Service
& Clinical Application
Alphabetical Listing