Glutamate Dehydrogenase (GLDH) Assay Kit                                                                                                                                                Price

Product Description: Glutamate dehydrogenase (GLDH), an important mitochondrial matrix enzyme integrating carbon and nitrogen
metabolism, catalyzes the reversible oxidative deamination of L-glutamate into free ammonia and alpha-ketoglutarate in all tissues. Since the
forward reaction generates a TCA cycle intermediate, GLDH is involved in energy homeostasis. In addition, kidney GLDH participates in acid/base
balance of blood by release of ammonia. In clinical diagnostics, increased circulating levels of GLDH signal toxic or hypoxic liver damage. Our non-
radioactive colorimetric GLDH assay is based on the reduction of the tetrazolium salt INT in a NADH-coupled enzymatic reaction to formazan,
which is water-soluble and exhibits an absorption maximum at 492 nm. The intensity of the red color formed is increased in the presence of
increased GLDH activity. The assay is simple and non-radioactive. Each kit is sufficient for 200 microassays. The assay solution should be kept in
aliquots at -80ºC, and will remain stable indefinitely at -80ºC.

Kit Components:

GLDH Assay Solution: 10 ml, store at -80ºC (for 200 assays)
10x GLDH Substrate: 1 ml, store at -80ºC
10x Cell Lysis Solution: 25 ml, store at 4ºC

MSDS: TX-100, DMSO, INT, acetic acid, Hepes

Related kits: L-Glutamate Assay

Citation:
Park et al
Identification of multiple Cryptococcal fungicidal drug targets by combined gene dosing and drug affinity responsive target stability screening
mBIO 7:e01073, 2016
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Biomedical Research Service
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Liver and heart tissue extracts were assayed
by GLDH Assay Kit. Enzyme activities were
expressed as units per mg standard. The
study shows that the liver exhibits a higher
GLDH enzyme activity than the heart.
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