Glucose-6-phosphatase (G6Pase) Assay Kit Price
Product Description: G6Pase is anchored in the membrane of the ER with the catalytic site facing the lumen, and converts glucose-6-phosphate
to glucose and inorganic phosphate (Pi). This activity of G6Pase plays an important role in gluconeogenesis and glycogenolysis. Expression of the
G6Pase gene is largely restricted to liver, kidney and to a lesser extent intestinal mucosa and pancreatic beta cells. Mutations in the catalytic
subunit of G6Pase or the ER membrane transporters can influence the availability of glucose, causing GSD Type Ia, Ib, and Ic. G6Pase deficiency
results in hypoglycemia and excessive intracellular accumulation of glucose-6-phosphate, which leads to elevated levels of lactate and uric acid.
The G6Pase enzyme activity assay kit is based detection of G6Pase-mediated release of Pi, which forms phosphomolybdate with the basic dye
Malachite Green (Anal. Biochem. 271:29,1999). Kit components are stable for at least 1 year if stored and handled properly.
G6Pase Buffer- 0.3 ml, store at 4ºC
G6Pase Substrate- 0.3 ml (150 wells), store at -80ºC
G6Pase Assay Solution- 15 ml, store at 4ºC (swirl bottle briefly prior to pipetting)
10x Cell Lysis Solution: 25 ml, store at 4ºC (swirl bottle briefly prior to pipetting)
MSDS: TX-100, HCl, Malachite Green, ammonium molybdate, MES
Related kits: HK Enzyme Assay, GAPDH Enzyme Assay, LDH Enzyme Assay, L-Lactate Assay, D-Lactate Assay, PDH Enzyme Assay, Glucose
Biomedical Research Service
& Clinical Application
Mouse liver tissue homogenates
were prepared from normal control
and MCD diet-fed mice. G6Pase
activities were assayed, showing that
G6Pase activitiy is significantly
reduced in the fatty liver.