Glucose-6-phosphate Dehydrogenase (G6PD) Assay Kit                                                                                                                             Price

Product Description: Glucose-6-phosphate dehydrogenase (G6PD), a soluble enzyme, catalyzes the rate-limiting and regulated step in the
pentose phosphate pathway, oxidizing glucose-6-phosphate with the production of NADPH, which is required for reductive biosynthesis and cellular
defense against microbial pathogens and reactive oxygen species. The enzyme is particularly important for maintaining the integrity of red blood
cells, and thus defective G6PD can cause hemolytic anemia in the presence of several precipitating factors (favism and infection). This
non-radioactive colorimetric G6PD assay is based on the reduction of the tetrazolium salt INT in a NADPH-coupled enzymatic reaction. The INT
reaction product is water-soluble and exhibits an absorption maximum at 492 nm. The intensity of the red color formed is increased in the presence
of increased G6PD activity. The assay solution should be kept at -80ºC for long-term storage (more than 1 month), and will remain stable
indefinitely at -80ºC.  

Kit Components:

G6PD Assay Solution: 10 ml (200 wells), store at -80ºC
10x Cell Lysis Solution: 25 ml, store at 4ºC

MSDS: TX-100, DMSO, INT, acetic acid, Hepes

Related kits: HK Enzyme Assay, GAPDH Enzyme Assay, LDH Enzyme Assay, L-Lactate Assay, PDH Enzyme Assay, Glucose Assay

Citation:
Zhu et al
Upregulation of non-canonical Wnt ligands and oxidative glucose metabolism in NASH induced by methionine-choline deficient diet.
Trends in Cell and Molecular Biology 13:47-56, 2018
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Biomedical Research Service
& Clinical Application
G6PD assays were performed
using heart extracts prepared from
normal hamster (F1B strain) and
cardiomyopathic hamster (TO2
strain). The assay shows that the
cardiomyopathic heart exhibits an
increased G6PD enzyme activity
due to sustained activation of the
endogenous cardiac repair
mechanism.
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